Why Check Your Vitamin D Levels?
Vitamin D is a essential fat-soluble nutrient that behaves like a hormone in your body. It is synthesized by the skin in response to sunlight and obtained from certain foods. Its most critical role is promoting the absorption of calcium in the gut, ensuring proper bone mineralization and preventing bone weakness (rickets in children, osteomalacia/osteoporosis in adults). The 25-Hydroxy Vitamin D test is the gold standard check to evaluate active vitamin D stores.
Test Price
₹1299 Only
Reporting Time
Next Day (24 Hours)
Fasting Needed
No (Random sample)
Sample Type
Blood (Serum clot activator)
Symptoms of Vitamin D Deficiency
Many people with vitamin D deficiency show no immediate symptoms. However, severe or chronic deficiency can lead to:
- Chronic bone aches, joint pain, and muscle weakness
- Frequent fatigue, low mood, or depressive feelings
- Delayed wound healing and frequent viral infections
- Hair loss and low bone mineral density (osteopenia)
Clinical Reference Ranges
| Level Range (ng/mL) | Clinical Interpretation |
|---|---|
| Less than 20 ng/mL | Deficient. High risk for bone thinning, fractures, and poor calcium metabolism. |
| 20 to 30 ng/mL | Insufficient. Sub-optimal level for skeletal and immune function. |
| 30 to 100 ng/mL | Sufficient. Healthy range supporting metabolic balance. |
| Greater than 100 ng/mL | Potential Toxicity. Typically caused by high-dose supplementation. |
How to Prepare for the Test?
Fasting is not required for a 25-Hydroxy Vitamin D test. You can eat, drink, and take your regular non-vitamin medications as usual. If you are taking high-dose biotin or multivitamin supplements containing biotin, check with your physician as it can interfere with certain immunoassays. Morning sample collection is preferred for convenience.